https://imcjms.com/public Ibrahim Medical College Journal of Medical Science https://imcjms.com/public/registration/journal_full_text/344 Sun, 10 May 2020 23:54:22 +0000 Abstract Background and objectives: Infertility is a global health problem including Bangladesh. Altered thyroid and prolactin levels have been implicated as a cause of infertility. The study was undertaken to find out the serum thyroid hormones and prolactin status in women with primary and secondary infertility. Methods: Women with primary and secondary infertility were enrolled. Fertile age-matched women were included as control. The anthropometric details (age, height and weight) were recorded. Overnight fasting blood sample was collected on 2nd day of menstrual cycle of the follicular phase. Serum thyroid stimulating hormone (TSH), free tri-iodothyronine (FT3) and free thyroxine(FT4) were measured by enzyme-linked immunosorbent assay (ELISA). Serum prolactin (PRL) was estimated by radioimmunoassay.  Results: A total of 150 women were enrolled in the study. Out of 150 women, 50 had primary and 50 had secondary infertility while 50 women were age-matched fertile women as control. The mean TSH levels of both infertility groups were significantly higher than that of fertile women. Regarding thyroid function, 24% and 28% of women with primary and secondary infertility had hypothyroidism respectively. The serum prolactin level was high in 42.9% and 50% of hypothyroid cases in primary and secondary infertility groups respectively. Conclusion: The study has demonstrated high occurrence of hypothyroidism with raised serum prolactin levels among infertile females emphasizing the importance of estimating both serum TSH and prolactin in infertility. IMC J Med Sci 2020; 14(1): 009. EPub date: 11 May 2020. DOI: https://doi.org/10.3329/imcjms.v14i1.47454 *Correspondence: Shamima Bari, Department of Physiology, Ibrahim Medical College, 1/A Ibrahim Sarani, Segunbagicha, Dhaka 1000, Bangladesh. Email: shamima.bari@yahoo.com   Introduction Infertility is an important health problem in Bangladesh. In Bangladesh, the rate of infertility has been reported as 4% to 15% [1-4]. The alteration of thyroid functions is associated with infertility [5-13]. Thyroid hormones, especially thyroid stimulating hormone (TSH), have been considered as an important component of infertility. Women with hyperprolactinemia have been found to have primary hypothyroidism. Thyroid dysfunctions interfere with numerous aspects of reproduction and pregnancy. Several articles have highlighted the association of hypothyroidism or hyperthyroidism with menstrual disturbance, anovulatory cycles, decreased productiveness and increased morbidity during pregnancy [8-10,14,15]. Hypothyroidism itself may contribute to infertility since thyroid hormones are necessary for the maximum production of both estradiol and progesterone [12,13]. Hence, it is necessary to screen serum thyroid hormones along with prolactin in women with infertility problems. Therefore, the present study was undertaken to determine the status of thyroid function in women with infertility.   Methods Study design: Women with primary and secondary infertility were included in the study. Equal number of age-matched apparently healthy fertile women was enrolled as control. Primary infertility denoted those women who had never conceived. Secondary infertility was defined as the same condition developing after initial phase of fertility that means the woman conceived previously but failed to conceive subsequently [12]. The fertility was defined as the capacity to conceive. Infertile women having husbands with normal semen analysis and those women with normal genitalia, uterus and adnexa were included. Women with tubal factor, congenital anomaly of urogenital tract and any obvious organic lesion or pelvic inflammatory diseases, and lactating women and also infertile women with subclinical hypothyroidism, secondary hypothyroidism were and subclinical hyperthyroidism, secondary hyperthyroidism were excluded from this study; only primary hypothyroidism and primary hyperthyroidism were included. The purpose and benefits of the study were explained to each participant and informed written consent was taken from each of them. A detailed medical, drug, personal, family, socio-economic histories were recorded in a predesigned questionnaire. The study was approved by the Institutional Ethical Review Committee. Collection of blood and estimation of biochemical parameters: Aseptically 5 milliliter of blood was collected from cubital vein of each participant. Blood was allowed to clot for 30-60 minutes at room temperature and then centrifuged at 3000 rpm for 5-10 minutes. The serum was separated and preserved at -20°C for estimation of serum TSH, FT4, FT3 and prolactin. The thyroid hormones were measured by enzyme-linked immunosorbent assay (ELISA) and serum prolactin was estimated by radioimmunoassay. The analysis was done within 2 weeks of blood collection. The normal range of serum, TSH, FT4, FT3 and prolactin were 0.3-4.0 mIU/L, 10.3-24.5 pmol/L, 2.3-6.3 pmol/L and 2-25 ng/ml respectively. Operational definition: The study population was categorized as (a) euthyroidism when the values of TSH and FT4 were within the normal range, (b) hypothyroidism when the TSH value exceeded 4.0 mIU/L and the FT4 value was normal or low, and (c) hyperthyroidism when the TSH value was <0.1 mIU/L or undetectable and normal or elevated FT3 or FT4 value [16,17]. Statistical Analysis: The data were analyzed by appropriate statistical tests namely, one way ANOVA, Tukey’s HSD post-hoc test and unpaired student’s t test and Z test.   Results A total of 150 women were included in the study. Out of 150 enrolled participants, 50 had primary (Group A) and another 50 had secondary infertility (Group B). Fifty age-matched apparently healthy fertile women were enrolled as control (Group C). The age range of the study population was from 23 years to 34 years and the mean age of different groups was almost similar and no statistically significant difference was observed. There was no significant difference of mean body mass index (BMI) between Group A and B. The mean serum levels of TSH, FT4, FT3 and prolactin of Group A, B and C are shown in Table-1. The mean serum TSH levels of women with primary (4.83±0.54 mIU/L)and secondary (6.40 ±0.59 mIU/L) infertility were significantly (p<0.001) higher than that of women with normal fertility (1.98±0.18 mIU/). The mean serum FT4 levels of women with primary (10.54±0.66 pmol/L) and secondary (7.64±0.44 pmol/L) infertility were significantly (p<0.005) lower than that of women with normal fertility (14.48±0.64 pmol/L). The mean serum FT3 levels of women with primary (4.12±0.32 pmol/L) and secondary infertility (3.9±0.23 pmol/L) were significantly (p=0.03 and p=0.001) lower than that of women with normal fertility (4.93±0.20 pmol/L). There was no significant difference of serum FT3 between Group A and B. Mean serum prolactin levels of women with primary (14.54±1.23 ng/ml) and secondary (15.36±1.02 ng/ml) infertility were significantly (p<0.05) higher than that of women with normal fertility (10.58±0.71 ng/ml). However, no significant difference was observed between women with primary and secondary infertility.   Table-1: Serum TSH, FT4, FT3 and prolactin levels of study population     According to the thyroid function status, 70% and 72% of the women having primary and secondary infertility were euthyroid respectively while 28% and 24% were suffering from hypothyroidism (Table-2). All the women in control group were euthyroid. Primary hyperthyroidism was present in 2% and 4% women with primary and secondary infertility (Table-2). The mean serum TSH level of hypothyroidism cases (7.97±0.72 mIU/L) of secondary infertility was significantly higher (p=0.02) compared to hypothyroidism cases of primary infertility group (5.14 ±0.85 mIU/L). High serum prolactin level was observed in 42.9% and 50% cases with hypothyroidism of women with primary and secondary infertility.   Table-2: Thyroid function status and prolactin levels in women with primary and secondary infertility     The Pearson’s correlation coefficient was calculated for serum TSH and prolactin in primary and secondary infertile women. In primary infertile women serum prolactin levels were significantly positively correlated with corresponding TSH levels (r =0.941, p <0.001; Figure-1). In secondary infertile women, serum prolactin levels also showed significantly positive correlation with serum TSH levels (r=0.915, p< 0.001, Figure-2). Hence, there was a strong association observed in primary and secondary infertile women with hyperprolactinemia and hypothyroidism.   Figure-1: Correlation of serum prolactin with TSH in primary infertile women     Figure-2: Correlation of serum prolactin with TSH in secondary infertile women     Discussion Thyroid dysfunction and alteration of prolactin levels have been reported as the cause of female infertility [8-10,15,18-23]. In the present study, hypothyroidism was found in 24%-28% women with primary and secondary infertility. Such pattern of thyroid dysfunction was also reported by several studies [8-15,18,20-22,24]. In hypothyroidism, increased thyrotropin-releasing hormone (TRH) production stimulates both TSH and prolactin secretion [23] and that leads to hyperprolactinemia and altered gonadotropin-releasing hormone (GnRH) secretion. 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