https://imcjms.com/public Ibrahim Medical College Journal of Medical Science https://imcjms.com/public/registration/journal_full_text/130 Sun, 06 Nov 2016 10:44:06 +0000 Ibrahim Med. Coll. J. 2009; 3(1): 9-12 Key words: LBW, Vitamin A, cord blood, neonates Address for Correspondence: Dr. Dipi Barua, Asstt. Prof. of Gynae & Obstetrics, Holy Family Red Cresent Medical College, Moghbazar, Dhaka   Materials and Methods This prospective randomized study was carried out in Maternity and Child Health Training Institute (MCHTI), Azimpur, Dhaka. The period of study was from January 2000 - July 2002. The study was conducted on 100 pregnant women (gestational age 38 – 40 wks) who visited the hospital for the purpose of delivery. The study included all 100 newborns of those mothers. Only those with single term pregnancy and having a normal delivery and with known gestational age were included in the study. Not included were those with medical diseases, obstetrical complications, and congenital malformations of infants. Mixed venous-arterial cord blood (about 3 ml) from the clamped umbilical cord (placental line), just after delivery (prior to expulsion of placenta) was collected directly into a glass tube. Serum was separated and immediately stored at –200C until Vitamin A level estimation was done. Maternal venous blood was drawn just before delivery and was processed similar to the cord serum. Estimation of Vitamin A was measured by using high performance liquid chromatography (HPLC). Neonate’s weight was taken within 20-30 minutes of delivery. Results It was found that 18% of the neonates had birth weights below 2500 gm and 82% were either 2500 gm or above. Thus majority of the neonates were within the normal range. Table 1 shows the mean (±SE) concentration of Vit A in cord and maternal serum. Cord serum Vit A level was found to be 58.27 ± 1.7µg/dl with a range of 4.93 - 102.04µg/dl, while maternal serum Vit A level was found to be (53.51 ± 1.48) µg/dl with a range of 17.19 – 89.17µg/dl. Table-1: Mean cord and maternal serum Vit A level of study population (n=100). Mean ± SE Cord serum Vit A (µg/dl) 4.93 – 102.04 53.5167 ± 1.4899   Table 2 shows the mean (±SE) concentration of serum Vitamin A level in cord serum by sex. Level of cord serum Vit A was found to be 59.05 ± 2.46µg/dl and 57.54 ± 2.43µg/dl for male and female neonates respectively. No significant sex difference was found in the concentration of cord serum A level. Table 3 shows that neonates having birth weight 2500 gm and above had cord Vitamin A level 57.84 ± 2.06 µg/dl and those with low birth weight i.e. below 2500 gm had 60.18 ± 2.03 µg/dl. No difference in relationship was found between birth weight and the level of Vit A in cord serum. Table 3 also shows that maternal serum Vit A level is 49.40 ± 3.04µg/dl in neonates weighing <2500gm and 54.49 ± 1.6µg/dl in case of neonates weighing 2500 gm or above having no significant difference. Table-2: Cord serum Vit A level of newborn neonates by sex (n=100) Sex Cord serum Vit A level Mean±SE Cord serum 48 4.09–64.02 Cord serum 52 52.64–62.43     Measures Level of Significance ³2500(Normal) 49.40±3.04 NS 60.18±2.03 NS     References 2.   WHO. Commentry on current world health organization used in perinatal statistics. Arch. Dis Child 1986; 61: 711-15. 4.   Khatoon SA. Low birth weight neonates: problem, how to solve it. Bangladesh J Child Health 1991; 15: 92-95. 6.   Shenai JP, Cytil F, Jhaveri A. and Stahlman MT. Plasma Vitamin A and retinol binding protein in premature and term neonates. J. Padiatr 1981;  99: 302-305. 8.   Shah R, Rajalakhsmi R, Bhatt RV, Hazra MN, Patel BC and Swmy NB. 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