https://imcjms.com Ibrahim Medical College Journal of Medical Science https://imcjms.com/registration/journal_full_text/479 Sat, 02 Sep 2023 12:31:34 +0000 Abstract Background and objectives: Reduction of weight improves different manifestations of polycystic ovary syndrome (PCOS). This study compared the effects of liraglutide plus metformin versus metformin alone on weight loss and metabolic profiles in obese women with PCOS. Methods: This open-label randomized controlled clinical trial consecutively recruited newly-diagnosed PCOS patients of reproductive age with obesity (body mass index ≥ 27.5 kg/m2). Following randomization into two equal groups, Group-1 received treatment with metformin 1000 mg daily alone while Group-2 was given metformin 1000 mg plus subcutaneous (SC) liraglutide 1.2 mg daily for 12 weeks. Anthropometric, biochemical and hormonal data and ovarian morphology were assessed at baseline and after 12 weeks. Clinical information and side effects were recorded every four weeks after initiation of the treatment. Glucose, lipids, and all hormones were analyzed by glucose oxidase, precipitation method, and chemiluminescent microparticle immunoassay respectively. Insulin resistance was measured by homeostatic model assessment (HOMA-IR). Results: Study included 30 participants comprising 15 for each group. Among 15 participants, 5 dropped out from the Group-1 and 1 dropped out from the Group-2. The final analysis was done among 24 participants (Gr-1: 10 and Gr-2: 14). Waist and hip circumference (WC, HC) significantly (p <0.05) decreased in patients treated with only metformin. Menstrual irregularity, BMI (body mass index), HC, systolic blood pressure (BP), 2h-OGTT glucose, fasting insulin, and HOMA-IR significantly (p < 0.05) decreased in the patients of Group-2 after 12 weeks compared to baseline status. Percentage changes of weight, BMI and HOMA-IR improved significantly (p < 0.05) in cases of Group-2 than those in Group-1. Side effects were though numerically higher in the Group-2 patients, but reduced with time. Conclusions: Addition of liraglutide with metformin was superior to metformin alone for lowering of BMI and insulin resistance among obese PCOS women with acceptable side effects. IMC J Med Sci. 2024; 18(1):002. DOI: https://doi.org/10.55010/imcjms.18.002 *Correspondence: Muhammad Abul Hasanat, Room# 1524, Level-15, Block-D, Bangabandhu Sheikh Mujib Medical University (BSMMU), Shahbag, Dhaka-1000, Bangladesh. ORCID iD: orcid.org/0000-0001-8151-9792; Email: aryansowgat@gmail.com   Introduction Polycystic ovary syndrome is a heterogeneous condition with a combination of reproductive, cutaneous, and metabolic features. Although its pathogenesis is largely unknown, hyperandrogenism and insulin resistance are the main determinants of clinical features [1, 2]. According to one hypothesis, PCOS symptoms develop when the body is unable to adjust to excess hepato-visceral fat acquired during the perinatal period. The central fat is pro-inflammatory and promotes both hyperandrogenemia and insulin resistance by secreting several types of adipocytokines. Ultimately, a vicious cycle is created between fat tissues and androgen-producing tissues, which perpetuate the typical features of PCOS [3]. Obesity affects around two-thirds of PCOS patients and is now considered a secondary cause of PCOS [4]. Several studies have shown amelioration of all features of PCOS after weight loss by lifestyle management and bariatric surgery [5,6]. Besides, obesity increases the manifestations of PCOS including hyperandrogenism, and reduces the pregnancy rate [7]. Management of PCOS is essentially symptomatic. Patients having metabolic features are often treated with insulin sensitizers. This use of insulin sensitizers in PCOS is off-level but evidence-based [8]. Metformin is a weight-neutral drug; however, along with the improvement of different manifestations, there is also a reduction of weight especially in patients with obesity [9,10]. Other weight-reducing drugs, especially glucagon-like peptide-1 receptor agonists (GLP-1-RAs) are attractive options. Recent studies have shown a wide spectrum of weight reductions in different obesity-related conditions including diabetes mellitus (DM), nonalcoholic fatty liver disease, obstructive sleep apnea, etc. by different types of GLP-1-RAs [11]. Liraglutide is a once-daily injectable GLP-1-RA that has achieved the approval of the Food and Drug Administration (USA) for the management of DM and obesity [12]. It works through a variety of mechanisms, including inhibiting the hypothalamus appetite center and delaying stomach emptying [13]. Its weight-loss impact is independent of its principal adverse effects, nausea, and vomiting [14]. Patients from South-Asian backgrounds have more metabolic manifestations and may benefit more from GLP-1-RAs [15]. The efficacy and safety of liraglutide in the management of PCOS are not adequately evaluated. This study compared the effects of metformin vs. metformin plus liraglutide in obese PCOS women. Both groups received advice on standard lifestyle management on metabolic and hormonal manifestations of PCOS.   Materials and methods The study was conducted at the PCOS Clinic of the Department of Endocrinology of Bangabandhu Sheikh Mujib Medical University (BSMMU) during the period of January 2018 to August 2019. The study was conducted according to the World Medical Association’ Declaration of Helsinki and the research protocol was approved by the Institutional Review Board (IRB) of BSMMU (No. BSMMU/2018/11032, Dated: 15/09/2018). Informed written consent was taken from all participants. Study type and population: This open-label randomized controlled clinical trial consecutively recruited newly-diagnosed PCOS patients of reproductive age (15 – 45 years) with obesity (body mass index (BMI) ≥27.5 kg/m2) [16]. PCOS was diagnosed on the basis of the Revised 2003 Rotterdam criteria [17]. Sample size was calculated by [n= 2σ2 (Z α+ Z β)2 / (μ1 - μ 2)2] formula where at Zα = 1.96, Zβ= 0.85 at 80% power, expected mean weight change in metformin plus liraglutide group: μ1= 6.5,  expected mean weight change between groups: μ2= 1.2  and σ = 6.8 (pooled standard deviation (SD) for each group) [18]. Participants having similar endocrine disorders, DM, chronic kidney disease, chronic liver disease, history of pancreatitis, personal or family history of medullary carcinoma of the thyroid, history of taking metformin, hormonal contraceptive, anti-obesity, or anti-androgen drugs within the last 6 months were excluded. Intervention: All the study participants were divided into two groups by a computer-generated random number chart.  Group-1 (metformin group) was treated with metformin 500 mg twice daily orally and the Group-2 (metformin + liraglutide group) was treated with metformin 500 mg orally twice daily plus subcutaneous injection of liraglutide 1.2 mg once daily for 12 weeks. To reduce the side effects of liraglutide, the participants of Group-2 was given 0.6 mg liraglutide once daily for the first two weeks; then increased to 1.2 mg once daily from the third week onward. Standard lifestyle advice including a weight-based diet, physical activity, and behavioral modifications was provided to both groups. All patients were educated about symptoms, signs, and management of side effects. Each patient was provided with medication according to her assigned category. Follow-up and investigations: At the first visit, anthropometric, clinical and biochemical data were recorded in a standard data sheet. The second visit was 2 weeks after the initiation of the study to increase the dose of liraglutide to 1.2 mg. Subsequent visits were made every four weeks from the initiation of the study. Clinical and anthropometric data were taken at every visit. Biochemical and imaging data were taken at the first and final visits. Weight (kilogram) and height (centimeter) were measured by calibrated bathroom scale and mounted measuring tape respectively to calculate BMI (kg/m2). WC (centimeter) was measured by measuring tape at the level of the umbilicus while HC was measured at the level of the largest lateral extension of the hip, both in a horizontal plane. Blood pressure was measured by a calibrated sphygmomanometer (mm-Hg). Hirsutism was measured by using the modified Ferriman-Gallwey (mFG) score. Acne was observed over the face. Acanthosis nigricans was checked on the neck, axilla, and groin. Amenorrhea was considered if a women missed at least three menstrual periods in a row  while oligomenorrheawas diagnosed when inter-menstrual intervals was greater than 35 days [19,20]. Menstruation occurring for consecutive two months was considered regular menstruation. Tests done in fasting state included: luteinizing hormone (LH), follicle-stimulating hormone (FSH), total testosterone (TT), fasting insulin, plasma glucose and lipid profile, followed by a standard 75 g oral glucose tolerance test (OGTT). Blood glucose was measured by the glucose oxidase method and serum LH, FSH, and TT were measured by chemiluminescent microparticle immunoassay at diagnosis during the follicular phase of the menstrual cycle. Total cholesterol (TC), triglycerides (TG), and high-density lipoprotein cholesterol (HDL-C) were measured by architect Plus Ci4100 automated analyzer. The homeostatic model assessment of insulin resistance (HOMA-IR) was calculated using the formula = (fasting glucose, mmol/L × fasting insulin, µU/mL) ÷ 22.5 [21]. Data analysis: The statistical analysis was done by SPSS software (version- 22.0). Numerical data were expressed in mean ± SD or median inter-quartile range (IQR) depending on their distribution. Qualitative data were expressed in frequency (%). There were no missing data. The percentage changes were calculated as follows: percentage changes = {(values after 3 months – values at baseline) ÷ values at baseline} × 100. For quantitative variables, comparisons between groups were done by independent samples t-test or Mann-Whitney U test, and within groups were done by paired t-test or Wilcoxon matched-pair signed rank test as appropriate. For qualitative variables, the associations between two groups were analyzed by Fisher’s exact test, and within groups were assessed by the McNemar test. Statistical significance for decision-making was set at two-tailed p-values below 0.05.   Results Study included 30 participants comprising 15 for each group. Among 15 participants, five dropped out from the Group-1 and 1 dropped out from the Group-2. The final analysis was done among 24 participants. The study flow chart is shown in Figure-1.     Figure-1: The study flow chart showing the enrollment, interventionand follow up scheme of the study participants   Table-1 shows that participants from both groups were not significantly (p>0.05) different with respect to age, personal history of subfertility, family history of PCOS, subfertility, obesity, hypertension, diabetes  as well as thyroid and prolactin statuses.   Table-1: Baseline characteristics of the study population (N= 24)     The anthropometric, clinical, biochemical, hormonal and imaging profiles of the study groups in relation to intervention are shown in Table-2. Patients in Group-2 had significantly higher levels of serum FSH (p=0.012) and HOMA-IR (p=0.042) levels than patients in the Group-1 before intervention. WC (p=0.032) and HC (p=0.028) decreased significantly in patients taking only metformin. Menstrual irregularity significantly (p=0.002) became regular in patients of Group-2. Also, BMI (p<0.001), HC (p=0.037), systolic BP (p=0.043), 2H-OGTT glucose (p=0.016), fasting insulin (p=0.012), and HOMA-IR (p=0.003) improved significantly in patients of Group-2 after intervention.   Table-2: Anthropometric, Clinical, biochemical, hormonal, and imaging characteristics of Group-1 (n=10) and Group-2 (n=14) study population in relation to intervention (N= 24)     Comparison of percentage changes of different variables shows BMI (p=0.023) and HOMA-IR (p=0.026) significantly decreased in Group-2 than that of Group-1 patients (Table-3). Percentage of weight loss was significantly (p=0.015) higher in the patients of Group-2 compared to Group-1 patients (mean difference 3 kg). Although, at least 5% weight loss was observed in 20% (2/10) and 57.1% (8/14) cases after intervention in Group-1 and Group-2 cases respectively, the p-value did not reach a significant level (p =0.104). Different types of side effects, especially gastrointestinal, were numerically higher in the Group-2 cases than those in Group-1. However, their frequency reduced with time (Table-4).   Table-3: Comparison of the percentage changes of anthropometric, clinical biochemical, and hormonal parameters between the study groups (N= 24)     Table-4: Adverse effects observed among the study population during the interventions (n= 24)     Discussion This open-label RCT showed the superiority of short-term (12 weeks) and low-dose of liraglutide (1.2 mg/ day) plus metformin therapy (1 g/day) over metformin (1 g/day) alone, along with lifestyle management, in reduction of BMI, and HOMA-IR among obese patients with PCOS. However, we did not find significant differences in other metabolic as well as hormone profiles between the study groups. Although the gastrointestinal side effects were initially higher in the metformin plus liraglutide group than in the metformin group, they reduced with time. In our study, when liraglutide was added to metformin, along with improvement of BMI and HC, menstrual irregularity, systolic blood pressure, 2H-OGTT glucose, fasting insulin, and insulin resistance also improved. Several meta-analyses suggest that liraglutide is superior to metformin in the improvement of metabolic manifestations [22,23]. When liraglutide is added to metformin, there is a synergistic effect [24]. Both WC and HC have significantly improved in patients of metformin group and are consistent with the findings of other studies conducted among PCOS patients with a BMI ≥25 kg/m2 [25]. However, we did not observe improvements in BMI and other endocrine and metabolic abnormalities which could be due to the short duration and lower doses of metformin. Patients of metformin plus liraglutide group additionally had reduction of weight and BMI by 3% and 1.2% respectively of the baseline than those in metformin group. A meta-analysis comprising three RCTs has reported similar weight loss and reduction of BMI with metformin plus liraglutide compared to metformin alone [26]. Rather than using absolute values, we used percentage changes as our study groups differed by BMI at baseline. Although higher percentages of our Group-2 patients achieved at least 5% weight loss than the Goup-1 cases (57.1% vs. 20.0%), the association was not statistically significant. Study from Slovenia also reported 5% weight loss in 22% cases among their study population receiving liraglutide and metformin [18]. It appears from our findings that people from South Asian backgrounds might respond better than the European population to GLP-1-RA [15]. The Slovenian study group has also shown in other studies that the weight loss response to liraglutide depends on the dose, metabolic status, and genetic polymorphism of GLP-1-RA [27-29]. We also found significant reduction in insulin resistance in patients receiving liraglutide plus metformin than the metformin alone. However, two similar studies did not find significant differences in HOMA-IR levels between cases of liraglutide plus metformin and the metformin groups [8,30]. A meta-analysis which included four RCTs, showed a reduction of both fasting glucose and insulin in patients having metformin plus liraglutide than the metformin alone, however, the values of HOMA-IR were not mentioned [26]. In our study, other metabolic manifestations, including glucose and lipid profile, changed similarly in both the study groups. Jensterle et al. also found similar findings except for a favorable effect on 2H-OGTT glucose levels in cases with metformin plus liraglutide [18]. However, their participants received a double dose of metformin than our study participants. In our study, 2H-OGTT glucose and systolic BP improved only in our Group-2 cases while Jensterle et al. did not find improvement in BP. We did not find any improvement in hormonal status within or between the study groups. Again, these findings are similar to the study conducted by Jensterle et al [18]. On the other hand, Xing et al. found significant improvement in free androgen index, LH, and progesterone levels in cases receiving combination of metformin and liraglutide compared to those in metformin group, despite a lack of improvement of any metabolic variables including BMI and HOMA-IR [30]. They also prescribed 2 gm of metformin per day for both groups. The menstrual cycle significantly improved only in metformin plus liraglutide group. While Xing et al. found improvement in the menstrual cycle in both groups while Jensterle et al. did not find it in any group [30,18]. Hirsutism, acne, acanthosis nigricans and PCOM almost remained similar to baseline indicating the requirement of a longer duration of treatment for significant improvement. Cases of metformin plus liraglutide group experienced more gastrointestinal side effects than those in metformin group. Nausea, loose motion, and vomiting were the most frequent side effects which were generally mild to moderate and subsided with time. The hypoglycemic events were absent. The short-term safety profile of using liraglutide in obese PCOS patients seemed to be acceptable. However, it is currently impossible to obtain precise estimates of the long-term risk of serious adverse effects such as pancreatitis or precancerous pancreatic lesion that has been claimed by some to be associated with GLP-1 based therapies. Although, a few patients complained of abdominal pain, these were non-specific, not associated with elevated lipase, and improved with symptomatic management. The main limitation of this study was lost to the follow-up of 33% of participants in the metformin group. One participant from both groups became pregnant, and others left the study from the metformin only group which might be due to the open-label nature of the study. In conclusion, this study demonstrated that when liraglutide was added to metformin, even at low dosages and for a short period of time, coupled with lifestyle management, metabolic parameters such as BMI and insulin resistance decreased significantly in obese PCOS women. Despite high cost and injectable form, liraglutide's effectiveness with acceptable side effects may be explored for the therapy of obesity in PCOS patients. Long-term study and higher dose may be required to ameliorate other metabolic, androgenic and hormonal abnormalities of obese PCOS patients.   Authors’ contribution AH, HB, MAH: Conception and design; AH, MSM, SA: Acquisition, analysis, and interpretation of data; All: Manuscript drafting and revising it critically   Competing interest The authors have nothing to declare.   Funding This study was partially supported by a Research grant from Research and Development, BSMMU, Novo Nordisk Pharma, and Beximco Pharmaceuticals Ltd., Bangladesh.   References 1.     Singh S, Pal N, Shubham S, Sarma DK, Verma V, Marotta F, Kumar M. Polycystic ovary syndrome: Etiology, current management, and future therapeutics. J Clin Med. 2023; 12(4): 1454. DOI: 10.3390/jcm12041454. 2.     Harada M. Pathophysiology of polycystic ovary syndrome revisited: Current understanding and perspectives regarding future research. Reprod Med Biol. 2022; 21(1): e12487. DOI: 10.1002/rmb2.12487. 3.     deZegher F, López-Bermejo A, Ibáñez L. Central obesity, faster maturation, and 'PCOS' in girls. Trends Endocrinol Metab. 2018; 29(12): 815-818. DOI: 10.1016/j.tem.2018.09.005. 4.     Morshed MS, Banu H, Akhtar N, Sultana T, Begum A,  Zamilla M, et al. Luteinizing hormone to follicle-stimulating hormone ratio significantly correlates with androgen level and manifestations are more frequent with hyperandrogenemia in women with polycystic ovary syndrome. J Endocrinol Metab. 2021; 11(1): 14-21. DOI:10.14740/jem716. 5.     Pasquali R, Gambineri A, Cavazza C, Ibarra Gasparini D, Ciampaglia W, Cognigni GE, et al. Heterogeneity in the responsiveness to long-term lifestyle intervention and predictability in obese women with polycystic ovary syndrome. Eur J Endocrinol.2011; 164(1): 53-60. DOI: 10.1530/EJE-10-0692. 6.     Escobar-Morreale HF, Botella-Carretero JI, Alvarez-Blasco F, Sancho J, San Millán JL. The polycystic ovary syndrome associated with morbid obesity may resolve after weight loss induced by bariatric surgery. J Clin Endocrinol Metab. 2005; 90(12): 6364-6369. DOI: 10.1210/jc.2005-1490. 7.     Cena H, Chiovato L, Nappi RE. Obesity, polycystic ovary syndrome, and infertility: A new avenue for GLP-1 receptor agonists. J Clin Endocrinol Metab. 2020; 105(8): e2695–2709. DOI: 10.1210/clinem/dgaa285. 8.     Rocha AL, Oliveira FR, Azevedo RC, Silva VA, Peres TM, Candido AL, et al. Recent advances in the understanding and management of polycystic ovary syndrome. F1000Res. 2019; 8: F1000 Faculty Rev-565. DOI: 10.12688/f1000research.15318.1.  9.    Aktar N, Hasanat MA, Banu H, Tuqan S, Mustari M, Sultana T, et al. Effect of metformin therapy over hormone profile in newly diagnosed polycystic ovary syndrome- A nine months randomized controlled trial. Am Res J Endocrinol. 2016; 1: 1-9. DOI:10.21694/2577-8412.17002. 10.  Akhtar N, Banu H, Morshed MS, Sultana T, Begum A, Hasanat MA. Effects of metformin in polycystic ovary syndrome: a randomized, double-blind, placebo-controlled study. IMC J Med Sci.2021; 15(2): 1-12. DOI: 10.3329/imcjms.v15i2.55808. 11.  Yazıcı D, YapıcıEser H, Kıyıcı S, Sancak S, Sezer H, Uygur M, et al. Clinical impact of glucagon-like peptide-1 receptor analogs on the complications of obesity. Obes Facts. 2023; 16(2): 149-163. DOI: 10.1159/000526808. 12.  Jensterle M, Rizzo M, Haluzík M, Janež A. Efficacy of GLP-1 RA approved for weight management in patients with or without diabetes: A narrative review. Adv Ther. 2022; 39(6): 2452-2467. DOI: 10.1007/s12325-022-02153-x. 13.  Ard J, Fitch A, Fruh S, Herman L. Weight loss and maintenance related to the mechanism of action of glucagon-like peptide 1 receptor agonists. Adv Ther. 2021; 38(6): 2821-2839. DOI: 10.1007/s12325-021-01710-0. 14.  Abu-Hamdah R, Rabiee A, Meneilly GS, Shannon RP, Andersen DK, Elahi D. Clinical review: The extrapancreatic effects of glucagon-like peptide-1 and related peptides. J Clin Endocrinol Metab. 2009; 94(6): 1843-52. DOI: 10.1210/jc.2008-1296. 15.  Lee MMY, Ghouri N, McGuire DK, Rutter MK, Sattar N. Meta-analyses of results from randomized outcome trials comparing cardiovascular effects of SGLT2is and GLP-1RAs in Asian versus White patients with and without type 2 diabetes. Diabetes Care. 2021; 44(5): 1236-1241. DOI: 10.2337/dc20-3007. 16.  WHO Expert Consultation. Appropriate body-mass index for Asian populations and its     implications for policy and intervention strategies. Lancet. 2004; 363(9403): 157-163. DOI: 10.1016/S0140-6736(03)15268-3. 17.  The Rotterdam ESHRE/ASRM‐sponsored PCOS consensus workshop group, Revised 2003 consensus on diagnostic criteria and long‐term health risks related to polycystic ovary syndrome (PCOS), Human Reproduction. Volume 19, Issue 1, January 2004, Pages 41–47. DOI:10.1093/humrep/deh098. 18.  Jensterle Sever M, Kocjan T, Pfeifer M, Kravos NA, Janez A. Short-term combined treatment with liraglutide and metformin leads to significant weight loss in obese women with polycystic ovary syndrome and previous poor response to metformin. Eur J Endocrinol. 2014; 170(3): 451-459. DOI: 10.1530/EJE-13-0797. 19.  Begum M, Das S, Sharma, HK. Menstrual disorders: causes and natural remedies. J Pharm Chem Biol Sci. 2016; 4: 307–320. 20.  Klein DA, Poth MA. Amenorrhea: an approach to diagnosis and management. Am Fam Physician. 2013; 87(11): 781–788. 21.  Matthews DR, Hosker JP, Rudenski AS, Naylor BA, Treacher DF, Turner RC. Homeostasis model assessment: insulin resistance and beta-cell function from fasting plasma glucose and insulin concentrations in man. Diabetologia. 1985; 28(7): 412-419. DOI: 10.1007/BF00280883. 22.  Tian D, Chen W, Xu Q, Li X, Lv Q. Liraglutide monotherapy and add on therapy on obese women with polycystic ovarian syndromes: A systematic review and meta-analysis. Minerva Med. 2022; 113(3): 542-550. DOI: 10.23736/S0026-4806.21.07085-3. 23.  Wang FF, Wu Y, Zhu YH, Ding T, Batterham RL, Qu F, Hardiman PJ. Pharmacologic therapy to induce weight loss in women who have obesity/overweight with polycystic ovary syndrome: A systematic review and network meta-analysis. Obes Rev. 2018; 19(10): 1424-1445. DOI: 10.1111/obr.12720. 24.  Jensterle M, Goricar K, Janez A. Metformin as an initial adjunct to low-dose liraglutide enhances the weight-decreasing potential of liraglutide in obese polycystic ovary syndrome: Randomized control study. Exp Ther Med. 2016; 11(4): 1194-1200. DOI: 10.3892/etm.2016.3081. 25.  Guan Y, Wang D, Bu H, Zhao T, Wang H. The effect of metformin on polycystic ovary syndrome in overweight women: A systematic review and meta-analysis of randomized controlled trials. Int J Endocrinol. 2020; 2020: 5150684. DOI: 10.1155/2020/5150684. 26.  Ge JJ, Wang DJ, Song W, Shen SM, Ge WH. The effectiveness and safety of liraglutide in treating overweight/obese patients with polycystic ovary syndrome: a meta-analysis. J Endocrinol Invest.2022; 45(2): 261-273. DOI: 10.1007/s40618-021-01666-6. 27.  Jensterle M, Kravos NA, Goričar K, Janez A. Short-term effectiveness of low dose liraglutide in combination with metformin versus high dose liraglutide alone in treatment of obese PCOS: randomized trial. BMC Endocr Disord. 2017; 17(1): 5. DOI: 10.1186/s12902-017-0155-9. 28.  Jensterle M, Kravos NA, Pfeifer M, Kocjan T, Janez A. A 12-week treatment with the long-acting glucagon-like peptide 1 receptor agonist liraglutide leads to significant weight loss in a subset of obese women with newly diagnosed polycystic ovary syndrome. Hormones (Athens). 2015; 14(1): 81-90. DOI: 10.1007/BF03401383. 29.  Jensterle M, Pirš B, Goričar K, Dolžan V, Janež A. Genetic variability in GLP-1 receptor is associated with inter-individual differences in weight lowering potential of liraglutide in obese women with PCOS: a pilot study. Eur J Clin Pharmacol. 2015; 71(7): 817-824. DOI: 10.1007/s00228-015-1868-1. 30.  Xing C, Zhao H, Zhang J, He B. Effect of metformin versus metformin plus liraglutide on gonadal and metabolic profiles in overweight patients with polycystic ovary syndrome. Front Endocrinol (Lausanne). 2022; 13: 945609. DOI: 10.3389/fendo.2022.945609.       Cite this article as: Hossain M, Banu H, Morshed MS, Afrine S, Hasanat MA. Short-term and low-dose liraglutide plus metformin decreased body mass index and insulin resistance more than metformin alone in obese women with polycystic ovary syndrome: An open-label randomized controlled study. IMC J Med Sci. 2024; 18(1):002. DOI: https://doi.org/10.55010/imcjms.18.002 2026 Ibrahim Medical College. All rights reserved.