IMC Journal of Medical Science
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Ibrahim Medical College Journal of Medical ScienceSelima SultanaShakil AkterMd. Ismail Khan https://www.imcjms.com/registration/journal_full_text/224
2017-06-04 12:47:33Original ArticleIbrahim Med. Coll. J. 2012; 6(2): 55-58
7 mmol/l on day 5 (72 hrs
after alloxan Inj.) were considered diabetic and selected for experimentation.
Both non-diabetic and diabetic treated groups (Gr II & IV) received Zingiber
officinale (ginger) juice (4 ml Kg–1 b.w., p.o.) for 10 days (day 2-day 11) through
Ryles tube. On Day 12, animals were sacrificed under light ether anaesthesia,
blood was collected by cardiac puncture and serum separated for estimation of
lipids.
The results suggest a significant anti-hyperlipidemic
action of Zingiber officinale (ginger) juice in alloxan induced diabetic
rats. The findings may be clinically significant and exploited.
Address for Correspondence: Dr. Selima Sultana, Assistant Professor,
Department of Pharmacology & Therapeutics, Ad-din Women’s Medical College,
2 Bara Magbazar, Dhaka-1217, e-mail: ark_udd@yahoo.com
Cardiovascular
diseases (coronary artery diseases, strokes and peripheral vascular diseases)
constitute the major causes of morbidity and mortality in diabetes mellitus.
Diabetic individuals have 2-4 times increased risk of clinical atherosclerotic
diseases.1 Hyperlipidemia is one of the most important
modifiable risk factors contributing to atherosclerosis in diabetes and may be
the result of unbalanced metabolic status of diabetes namely hyperlipidemia and
insulin resistance.2
The
present study was undertaken to investigate the effect of fresh ginger juice on
lipid profile (hyperlipidemia) in alloxan induced diabetic rats compared to
non-diabetic controls.
Materials and Methods
The fresh rhizome of Z. officinale (ginger) was obtained
from local market. 1 Kg of fresh rhizome were crushed, then squeezed in muslin
cloth to obtain the juice using the method of Akhain et al.4 Sodium benzoate (0.5%) was
added as preservative. The juice was stored in the refrigerator at 2-80C in a well-closed glass container.
Animals
After 24 hrs fasting, rats (group III & IV) were injected
alloxan 150 mg Kg–1 b.w.ip
on Day 2 of the study. Fasting blood glucose levels were estimated on Day 1
(before Inj. alloxan), on Day 5 (72 hrs after Inj. alloxan) and on Day 12 of
the experimental study. Blood glucose was estimated by placing a test strip in
the glucometer (ACCU-CHEK, Roche diagnostic GmbH). A drop of blood was
collected by asceptically cutting the tail at the tip (0.1 cm) with shrap
sterile blade and then applying the drop of blood to the test area of the
strip. Rats with blood glucose of >7 mmol/l on Day 5 (i.e 72 hrs after Inj.
alloxan) were considered diabetic and selected for experimentation.
Experimental design
The
results are presented as mean±SD. Unpaired ‘t’ test was performed and p value
<0.05 was considered as statistically significant.
Results
i. The mean±SD of blood
glucose (mmol/l), in normal (non-diabetic) rats (group I) on day 2 and day 12
of the study were 5.40±0.76 and 5.45±0.76 respectively, while in normal
(non-diabetic) treated (ginger juice 4 ml Kg–1 b.w. for 10 days) rats (group II) were
5.45±0.76 and 5.47±0.59 respectively. The differences between two group (I vs
II) were not statistically significant (p <0.05) suggesting that ginger
juice did not lower blood glucose level in normal (non-diabetic) rats.
iii.The mean±SD of blood
glucose (mmol/l), of diabetic control rats (group III) and of diabetic treated
(ginger juice 4 ml Kg–1 b.w.
for 10 days) rats (group IV) on day 12 of the study were 8.52±0.68 and
7.52±0.42 respectively. The differences between two groups (III vs IV) were
statistically significant (p <0.01) suggesting that treatment of diabetic
rats with ginger juice produced significant decrease in blood glucose level.
Table-1: Effects
of Zingiber officinale (ginger) juice on lipid profile in normal (non-diabetic)
rats
i. Effect of Zingiber
officinale (ginger) juice on lipid profile in normal (non-diabetic) rats
ii. Effect of Zingiber
officinale (ginger) juice on lipid profile in diabetic rats
The results are shown in
Table-2.
Table-2: Effect
of Zingiber officinale (ginger) juice on lipid profile in diabetic rats
The
present study was undertaken to investigate the effects of Zingiber
officinale (ginger) juice on lipid profile in alloxan induced diabetic rats
compared to normal non-diabetic controls. Injection of alloxan (150 mg Kg–1 b.w,i.p) produced marked
hyperglycemia and hyperlipidemia (increased Tol. chol, LDL-chol & TG and
decreased HDL-chol). Treatment with Zingiber officinale (ginger) juice
(4 ml Kg–1 b.w,
p.o) for 10 days to alloxan induced diabetic rats produced significant blood
glucose and lipid lowering (decreased Tol. chol, LDL-chol & TG and
increased HDL-chol) effects. However treatment of ginger juice for 10 days to
normal non-diabetic rats did not produce significant lipid lowering effects;
thus suggesting a significant anti-hyperlipidemic action for Zingiber
officinale (ginger) juice in alloxan induced diabetic rats. The results are
in agreement with those of previous studies2,5,6,8 who showed similar lipid
lowering effects of Z. officinale in different experimental animal
models.
1. Nathan DM, Meigs J,
Singer DE. The epidemiology of cardiovascular disease in type 2 diabetes
mellitus. Lancet 1997; 350(1): SI4-9.
3. Bhandari U, Grover JK.
Effect of ethanolic extract of ginger on hyperglycemic rats. Int. J.
Diabetes 1998; 6: 95-96.
5. Fuhrman B, Rosenblat M,
Hayek T, Coleman R, Aviram M. Z. officinale extract consumption reduces plasma
cholesterol, inhibits LDL oxidation and attenutes development of
atherosclesosis in atheroscherotic, apolipoprotein E-deficient mice. J. Nutr
2000; 130: 1124-1131.
7. Park KK, Chun KS, Lee JM,
Lee SS, Surh YJ. Inhibitory effects of 6-gngerol, a major pungent principle of
ginger on phorhol ester-induced inflammation, epidermal ornithine decarboxylase
activity and skin tumor promotion in ICR mice. Cancer Letters 1998; 129:
139-144.
9. Katiyar SK, Agarwal R,
Mukhtae H. Inhibition of tumor promotion in SENCAR mouse skin by ethanol
extract of Zingiber officinale rhizome. Cancer Research 1996; 56:
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